Proinflammatory Cytokines Associated with InvA and PagC Genes of Salmonella typhi Isolated from Patients Undergoing Cholecystectomy
نویسندگان
چکیده
Objective: Cholecystectomy is the surgical removal of gallbladder. The gallbladder often colonized by Salmonella during typhoid fever. association between cytokine and virulence genes not well known. current study aims to identify proinflammatory cytokines, TNF-α IFN-γ, two genes, InvA PagC, extracted from S. typhi isolates obtained patients undergoing cholecystectomy.
 Materials Methods: One hundred fifty clinical specimens, including tissues blood, were collected cholecystectomy at AL-Sadder Medical City AL-Furat General Hospital/Iraq December 2019 September 2020. A Monoplex PCR technique was used detect an enzyme-linked immunosorbent assay measure serum levels IFNγ.
 Results: From a total 66 isolates, prevalence PagC (84.8% 69.6%) respectively. Even after adjusting for potential confounding factors, INF-γ statistically significantly higher in detected compared (p-value<0.001), but there no significant difference (p-value > 0.05).
 Conclusion: typhi, especially may be considered potent inducer secretion. Further studies are needed present patient‘s additional course according level.
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ژورنال
عنوان ژورنال: Siriraj Medical Journal
سال: 2022
ISSN: ['2629-995X']
DOI: https://doi.org/10.33192/smj.2022.97